Organize Safety Evaluation of High Power Medical Holmium Laser Therapeutic Machine on Canine Bladder Lithotripsy

Keywords:holmium, laser, , therapeutic, machine,  Time:20-07-2015

JIA Ziming1, SUN Fanzhong 1, YANG Wenxiang1, YI Ping 1, HUANG Heng 2, LIU Jiafa1, TANG Lijun1(1. Hubei Provincial Key Laboratory for Applied Toxicology, Hubei Provincial Academy of Preventive Medicine, Wuhan 430079, China; 2. Hefei Han’s Curestar Laser Debice Co., Ltd., Hefei 230088, China) 1
Abstract：To evaluate the safety of treating bladder calculi with holmium laser, we set up a bladder calculus model, which is low time-consuming and has no damage to the bladder tissue, by using cystoscope sheath to implant human stones into the bladders of six female Beagle dogs. Then we performed bladder lithotripsy using a medical holmium laser therapeutic machine (HANS－H100), which had output power of 100 W and worked in cooperation with cystoscope, and hence con-ducted  medical  experiments  for  organize  safety  evaluation.  After  the  treatment,  all  the  six  dogs  behaved  normally  and showed no abnormity in eating, drinking, breathing, and external reactions after lithotripsy, though there was damage to either urethra or bladder according to the hematuria observed. However, this damage was reversible, since the dogs’ urine returned to normal 14 days after the lithotripsy. Different degrees of injuries appeared in the bladder mucosa in the 24th hour after treatment, such as degeneration of epithelial cell necrosis, mucosal edema, inflammatory cells infiltration, mus-cle layer edema between muscle fibers, and inflammatory cell infiltration. In addition, mucosal epithelial cell shedding, mucosal edema, lamina propria connective tissue hyperplasia of granulation became the main symptoms in the 7th day. The bladder tissue structure recovered in the 14th day after the lithotripsy, indicating that the damage was also reversible. The experiments proved that the high-power medical holmium laser therapy machine was safe and effective for treating bladder stones.
Key words：holmium laser ; therapeutic machine ; Beagle dogs; bladder stones; new model; lithotripsy; organize safety